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目的：以白血病多药耐药细胞系Ｋ５６２／Ａ０２为对象，验证苦参碱能否逆转其对柔红霉素的耐药性。方法和结果：经ＭＴＴ法发现苦参碱５０ｍｇ／Ｌ能增加Ｋ５６２／Ａ０２对柔红霉素的敏感性，使柔红霉素半数抑制浓度（ＩＣ５０）由１４９２ｍｇ／Ｌ降至８２９ｍｇ／Ｌ，部分逆转了Ｋ５６２／Ａ０２对柔红霉素耐药性，逆转倍数为１８０倍。经流式细胞仪，免疫组化、逆转录—多酶链式反应证明苦参碱逆转Ｋ５６２／Ａ０２耐药性机制主要为：苦参碱降低多药耐药基因ｍｄｒ－１的ｍＲＮＡ表达，使细胞膜上Ｐ－１７０糖蛋白量减少，从而降低经Ｐ－１７０糖蛋白介导的细胞内柔红霉素外排能力，细胞内柔红霉素浓度升高，增强了Ｋ５６２／Ａ０２对柔红霉素敏感性。而苦参碱对Ｋ５６２／ｓ敏感细胞系无上述作用
Objective: To verify whether matrine can reverse its resistance to daunorubicin in a multidrug-resistant leukemia cell line K562/A02. METHODS AND RESULTS: MTT assay showed that matrine 50 mg/L increased the sensitivity of K562/A02 to daunorubicin, reducing the half-inhibitory concentration (IC50) of daunorubicin from 14 to 92 mg/L to 8 to 29 mg. /L partially reversed K562/A02 resistance to daunorubicin, with a reversal multiple of 180. By flow cytometry, immunohistochemistry, reverse transcription-multiplexase chain reaction demonstrated that the matrine reversal mechanism of K562/A02 drug resistance is mainly: matrine reduces multidrug resistance gene mdr-1 mRNA expression, so that The amount of P-170 glycoprotein on the cell membrane is reduced, thereby reducing intracellular daunorubicin efflux ability mediated by P-170 glycoprotein, increasing the intracellular concentration of daunorubicin, and enhancing K562/A02 against rhodoerythrin Susceptibility. Matrine does not have the above effect on K562/s sensitive cell lines