论文部分内容阅读
目的 建立玉屏风颗粒中黄芪甲苷的 RP- HPL C定量测定方法。方法 玉屏风颗粒经甲醇超声提取 ,水浴蒸干 ,用正丁醇溶解 ,氨试液洗涤后 ,再蒸干正丁醇溶液 ,并加甲醇溶解、定容 ,制成供试液 ,注入高效液相色谱仪进行测定。色谱柱为 No Va- pak C1 8柱 ;流动相为乙腈 -水 (1∶ 2 .5 ) ,流速 :1.0 ml/ min;检测波长为 2 0 3nm ;检测温度 :40℃ ;纸速 :0 .2 5 cm/min。结果 黄芪甲苷的保留时间约为 12 .5 m in。其在 5 .195μg～ 5 1.95μg范围内线性关系良好 ,回归方程为 Y=42 0 2 8.9X+16 935 .6 (r=0 .9996 ) ,平均回收率为 97.0 2 % (n=6 ) ,RSD=1.0 8%。结论 该法灵敏、快速、准确 ,可作为玉屏风颗粒中黄芪甲苷的含量测定方法
Objective To establish a method for quantitative determination of RP-HPL C in astragaloside IV in Yupingfeng particles. Methods Yupingfeng particles were extracted by ultrasonic extraction with methanol, the water bath was evaporated to dryness, dissolved with n-butanol, and washed with ammonia solution. The n-butanol solution was then evaporated to dryness, and dissolved in methanol to obtain a test solution. Chromatography analyzer for the determination. The column was a No Va-pak C18 column; the mobile phase was acetonitrile-water (1:2.5), the flow rate was 1.0 ml/min, the detection wavelength was 20.3 nm, the detection temperature was 40°C, and the paper speed was 0. 25 cm/min. Results The retention time of astragaloside was about 12.5 m in. The linearity was good in the range of 5.195μg～5 1.95μg. The regression equation was Y=42 0 2 8.9X+16 935 .6 (r=0.9996) and the average recovery was 97.0 2 % (n=6). ,RSD=1.0 8%. Conclusion This method is sensitive, rapid and accurate and can be used as a method for determining the content of astragaloside IV in Yupingfeng granules.