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目的 :研究三七总皂苷(PNS)对脂多糖(LPS)诱导的大鼠心肌细胞肥大的保护作用。方法:体外培养新生1~2d的SD大鼠乳鼠心肌细胞48 h后,设正常对照组、脂多糖1mg/L模型组、脂多糖+三七总皂苷12.5、25、50 mg/L组。考马斯亮蓝法测细胞的蛋白含量;计算机图像分析系统测量细胞体积;酶联免疫吸附实验(ELISA)检测细胞肿瘤坏死因子(TNF-α)的含量;以Fura-2/AM为荧光探针,采用Till阳离子测定系统,观测细胞内[Ca2+]i瞬间变化。结果:与正常对照组相比,脂多糖1 mg/L可使心肌细胞蛋白含量明显增多,体积显著增大,释放的TNF-α含量显著增加,心肌细胞内[Ca2+]i瞬间峰值增大。与脂多糖模型组相比,预加入12.5、25、50 mg/L的三七总皂苷均可抑制心肌细胞蛋白含量增多,体积增大,同时细胞产生的TNF-α含量明显降低,抑制了细胞内[Ca2+]i瞬间变化幅度增大。结论:三七总皂苷对脂多糖诱导的大鼠乳鼠心肌细胞肥大有良好的保护作用,其机制可能与抑制TNF-α的产生,降低[Ca2+]i有关。
Objective: To study the protective effects of Panax Notoginseng Saponins (PNS) on cardiomyocyte hypertrophy induced by lipopolysaccharide (LPS) in rats. Methods: Cardiomyocytes of neonatal SD rats were cultured for 48 h in vitro. Normal control group, 1 mg / L lipopolysaccharide group, 12.5, 25 and 50 mg / L lipopolysaccharide + total notoginseng saponins were prepared. Coomassie brilliant blue method was used to measure the protein content of cells; computer image analysis system was used to measure the cell volume; enzyme-linked immunosorbent assay (ELISA) was used to detect the content of tumor necrosis factor (TNF-α); Fura- Till cationic assay system was used to observe intracellular [Ca2 +] i transient changes. Results: Compared with the normal control group, lipopolysaccharide (1 mg / L) significantly increased the protein content of cardiomyocytes, significantly increased the volume of TNF-α, and significantly increased the instantaneous peak of [Ca2 +] i in myocardial cells. Compared with the model group, pretreatment with Panax notoginseng at concentrations of 12.5, 25 and 50 mg / L could inhibit the increase of cardiomyocyte protein and volume, and at the same time, the content of TNF- Within the [Ca2 +] i instantaneous increase in amplitude. Conclusion: Panax notoginseng Saponins can protect rat cardiomyocytes from hypertrophy induced by lipopolysaccharide. The mechanism may be related to inhibiting the production of TNF-α and decreasing [Ca2 +] i.