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为了研究丙型肝炎病毒 (HCV)感染外周血单个核细胞 (PBMC)对丙型肝炎的影响 ,运用非同位素原位杂交法 (NISH)和链酶亲和素 -生物素 (SABC)法分别检测 2 0例慢性丙型肝炎患者 PBMC中的 HCV- RNA及其 T淋巴细胞亚群 ,同时检测这些患者的肝功能指标。结果显示 8例 (4 0 .0 % ) HCV患者的 PBMC中的 HCV- RNA呈阳性结果。HCV- RNA呈散在颗粒状或弥漫分布 ,主要位于胞浆中。 HCV- RNA阳性组和阴性组的 CD+4 T细胞比例低于正常对照组 ,CD+8T细胞比例高于正常对照组 ,CD+4 / CD+8比值下降 ;而 HCV- RNA阳性组的 CD+4 T细胞比例则又低于阴性组 ,CD+8T细胞比例高于阴性组 ,CD+4 / CD+8比值出现倒置 (P<0 .0 1)。两组的肝功能检查结果之间无显著差异 (P>0 .0 5 )。提示 HCV感染 PBMC后引起 T淋巴细胞亚群发生变化 ,使 CD+4 和 CD+8T细胞功能下降或不能很好地协调作用 ,从而成为 HCV持续感染的重要原因之一。 HCV感染 PBMC对肝脏炎症程度的影响可能不大
To investigate the effect of peripheral blood mononuclear cells (PBMC) infected with hepatitis C virus (HCV) on Hepatitis C, non-isotopic in situ hybridization (NISH) and streptavidin-biotin (SABC) 20 cases of chronic hepatitis C in patients with HCV-RNA and T-lymphocyte subsets, while the detection of liver function in these patients. The results showed positive HCV-RNA in PBMC from 8 patients (40.0%) of HCV patients. HCV-RNA was scattered in granular or diffuse distribution, mainly in the cytoplasm. The proportion of CD + 4 T cells in HCV-RNA positive group and negative group was lower than that in normal control group, the proportion of CD + 8 T cells was higher than that in normal control group, and the ratio of CD + 4 / CD + 8 was decreased. +4 T cells was lower than the negative group, the proportion of CD + 8 T cells was higher than the negative group, and the ratio of CD + 4 / CD + 8 was upside down (P <0.01). There was no significant difference between the two groups in liver function test results (P> 0.05). It is suggested that the infection of PBMC by HCV causes the changes of T lymphocyte subsets, and the function of CD + 4 and CD + 8T cells is decreased or not well coordinated, which is one of the important reasons for persistent HCV infection. The effect of HCV-infected PBMC on the degree of liver inflammation may not be significant