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目的:建立高效液相色谱测定山楂、丹参药对提取物中原儿茶醛、金丝桃苷、迷迭香酸、紫草酸、丹酚酸B、槲皮素6个成分含量的方法。方法:采用Phenomsil-C18(4.6 mm×250 mm,5μm)色谱柱,流动相乙腈-0.5%磷酸水,梯度洗脱,流速1.0 mL.min-1,检测波长300 nm,柱温30℃。结果:原儿茶醛、金丝桃苷、迷迭香酸、紫草酸、丹酚酸B、槲皮素6个成分的进样量分别在0.021 58～0.107 9μg(r=0.999 4),0.102 6～0.513 0μg(r=0.999 2),0.157 5～0.787 5μg(r=0.999 0),0.245 6～1.228μg(r=0.999 1),2.600～13.00μg(r=0.999 0),0.085 1～0.425 6μg(r=0.999 1)与色谱峰面积呈良好的线性关系。原儿茶醛、金丝桃苷、迷迭香酸、紫草酸、丹酚酸B、槲皮素6个成分加样回收率(n=6)分别为99.1%,99.3%,100.2%,99.2%,98.9%,99.2%,RSD均<3%。结论:方法可为山楂、丹参药对提取物的质量控制提供参考。
Objective: To establish a method for the determination of six components of protocatechuic aldehyde, hyperoside, rosmarinic acid, lithospatrose, salvianolic acid B and quercetin in hawthorn and salvia miltiorrhiza Bunge by HPLC. Methods: The mobile phase consisted of acetonitrile-0.5% phosphoric acid solution with a gradient of 1.0 mL.min-1 at a flow rate of 1.0 mL.min-1 on a Phenomsil-C18 column (4.6 mm × 250 mm, 5 μm). The detection wavelength was 300 nm and the column temperature was 30 ℃. Results: The protocatechuic aldehyde, hyperoside, rosmarinic acid, lithospermic acid, salvianolic acid B, quercetin 6 components in the injection volume were 0.021 58 ~ 0.107 9μg (r = 0.999 4), 0.102 (R = 0.999 1), 2.600 ~ 13.00 μg (r = 0.999 0), 0.085 1 ~0.4252 (r = 0.999 2), 0.245 6 ~ 1.228 μg 6μg (r = 0.999 1) and the chromatographic peak area showed a good linear relationship. The recoveries (n = 6) of protocatechualdehyde, hyperoside, rosmarinic acid, lithospermate, salvianolic acid B and quercetin were 99.1%, 99.3%, 100.2% and 99.2 %, 98.9%, 99.2%, RSD <3%. Conclusion: The method can provide reference for the quality control of hawthorn and salvia miltiorrhiza.