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目的通过观察软肝升白颗粒对肝纤维化小鼠肝脏肝细胞生长因子及其C-Met mRNA表达的影响,探讨其抗肝纤维化的作用机制。方法 80只BABL/c小鼠给予0.03%硫代乙酰胺饮用水喂养20周,制备肝纤维化动物模型[1]。随机分为4组,每组20只。A组小鼠给予软肝升白颗粒11.2 g/(kg.d),B组小鼠给予软肝升白颗粒5.6 g/(kg.d),C组小鼠给予鳖甲软肝片1.12 g/(kg.d),D组分别给予等体积的生理盐水,每天灌胃1次,另外,20只小鼠作为正常对照组(E组),处理方法同D组。分别在4周和8周时,各处死小鼠10只,检测血清肝功能指标,各组取肝组织HE染色肝组织学观察,同时应用RT-PCR技术检测肝组织中肝细胞生长因子(HGF)及其受体C-Met mRNA的表达量。结果治疗前后肝功能和组织学均存在显著性差异。治疗组HGF/C-Met mRNA表达显著高于对照组(P<0.05)。结论肝纤维化发生后,软肝升白颗粒能使HGF/C-Met mRNA高表达。
Objective To observe the effects of Ruan-sheng she Bai granule on the expression of liver hepatocyte growth factor and its C-Met mRNA in liver fibrosis mice and to explore the mechanism of action of Ruan-sheng sheng Bai on hepatic fibrosis. Methods Eighty BABL / c mice were given 0.03% thioacetamide drinking water for 20 weeks to prepare liver fibrosis animal model [1]. Randomly divided into 4 groups, each group of 20. A group of mice given soft liver lightening particles 11.2 g / (kg.d), B group of mice given soft liver lightening particles 5.6 g / (kg.d), C group mice were given Biejia Ruangan tablets 1.12 g /(kg.d), D group were given an equal volume of saline, gavage once a day, in addition, 20 mice as a normal control group (E group), with the treatment of D group. At 4 weeks and 8 weeks, 10 mice were sacrificed to detect the serum liver function indexes. The liver tissues of each group were harvested for liver histology. RT-PCR was used to detect the expression of hepatocyte growth factor (HGF) ) And its receptor C-Met mRNA expression. Results Before and after treatment, there were significant differences in liver function and histology. The expression of HGF / C-Met mRNA in the treatment group was significantly higher than that in the control group (P <0.05). Conclusion The occurrence of liver fibrosis, Ruanghuanglibai particles HGF / C-Met mRNA expression.