论文部分内容阅读
目的研究NaCl胁迫对披针叶黄华Thermopsis laceolata愈伤组织生长及生理特性的影响,拟从细胞水平上探讨其适应盐环境的生理机制。方法在附加0.2%~1.2%NaCl的继代培养基上对愈伤组织进行胁迫培养,测定其中的可溶性糖、可溶性蛋白质、脯氨酸、丙二醛(MDA)量及超氧化物歧化酶(SOD)、过氧化物酶(POD)和过氧化氢酶(CAT)的活性,并对其进行分析。结果当NaCl小于或等于0.8%时,随盐离子质量分数的增加,愈伤组织生长量下降,但与对照均无显著差异,而当NaCl大于0.8%时,愈伤组织生长极为缓慢;可溶性糖和脯氨酸量随NaCl质量分数的升高总体呈先上升后下降趋势,但均高于对照;可溶性蛋白质、SOD、CAT和POD酶活性随NaCl质量分数升高呈先上升后下降趋势;丙二醛量在0.8%NaCl及以下时积累缓慢,而NaCl高于0.8%时急剧增加。结论披针叶黄华愈伤组织具有适应一定质量分数(≤0.8%NaCl)盐渍生境的能力,其在受到盐胁迫时可以通过增加可溶性渗透调节物质如脯氨酸、可溶性糖类以降低渗透势及增强抗氧化酶活性来缓解盐伤害。
Objective To study the effects of NaCl stress on the growth and physiological characteristics of Thermopsis laceolata callus, and to explore the physiological mechanism of salt stress adaptation at the cellular level. Methods Callus culture was carried out on subcultures supplemented with 0.2% -1.2% NaCl. Soluble sugar, soluble protein, proline, malondialdehyde (MDA) and superoxide dismutase SOD), peroxidase (POD) and catalase (CAT) were measured and analyzed. Results When NaCl was less than or equal to 0.8%, the growth of callus decreased with the increase of salt ion concentration, but no significant difference was found between the two treatments. When the NaCl concentration was more than 0.8%, the callus growth was very slow. Soluble sugar And proline increased first and then decreased with the increase of NaCl concentration, but both were higher than the control. The activities of soluble protein, SOD, CAT and POD increased first and then decreased with the increase of NaCl concentration. Accumulation slowed down at 0.8% NaCl and below and sharply increased at NaCl above 0.8%. Conclusions The callus of F. lanceolata callus possesses the ability to adapt to the saline habitat with certain mass fraction (≤0.8% NaCl), which can reduce the osmotic potential by increasing the soluble osmolytic substances such as proline and soluble carbohydrates Enhances antioxidant enzyme activity to alleviate salt damage.