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For the control of stem rot in Dendrobium officinale,symptoms were observed and the pathogen was isolated and identified from April 2013 to August2015. The pathogen only infected the stem of D. officinale,resulting in death of stem above the lesion. Strain TP-1 accounted for 65. 7% of isolated colonies. After inoculation of TP-1 for 14 d,water-soaked necrotic lesions about 3 mm in diameter were observed,which were similar to natural infections,and the original strain was obtained after re-isolation. Morphology and sequencing identification confirmed that TP-1 was Lasiodiplodia theobromae. This is the first report of stem rot in D. officinale caused by L. theobromae in China.
For the control of stem rot in Dendrobium officinale, symptoms were observed and the pathogen was isolated and identified from April 2013 to August 2015. The pathogen only infected the stem of D. officinale, resulting in death of stem above the lesion. Strain TP-1 after inoculation of TP-1 for 14 d, water-soaked necrotic lesions about 3 mm in diameter were observed, which were similar to natural infections, and the original strain was obtained after re-isolation . Morphology and sequencing identification confirmed that TP-1 was Lasiodiplodia theobromae. This is the first report of stem rot in D. officinale caused by L. theobromae in China.