Expression of Avian Reovirus (ARV) σA Protein in HEK293T Cells

• 作 者: Ren Hongyu Xie Zhixun Xie Liji
• 机 构: GuangxiKeyLaboratoryofVeterinaryBiologicalTechnology
• 出 处: 动物与饲料科学:英文版
• 发表日期: 2019年5期
• 关键词: ARV σA gene Cloning σA PROTEIN EUKARYOTIC EXPRESSION PLASMID
• 基金项目: Supported by Guangxi Science Base and Talents Special Program(AD17195083),Guangxi Science Great Special Program(AA17204057),National Natural Science Foundation of China(31660715,31160512),Science and

[Objective]The paper was to construct eukaryotic expression vector of Avian reovirus(ARV)σA gene and expressσA protein accurately in HEK293T cells.[Method]The specific primers of ARVσA gene were designed according to the gene sequence of ARV S2 gene in Ge