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目的:观察血小板经不同mPEG修饰后功能变化情况,并分析其原因。方法:分别用mPEG-BTC、mPEG-SPA对血小板进行化学修饰;流式法检测修饰前后血小板CD62P荧光强度变化;并对血小板的聚集、黏附及释放功能进行检测。结果:经mPEG-BTC、mPEG-SPA修饰后,血小板CD62P表达率由修饰前的(12.77±2.87)%分别降至0和(4.12±1.28)%;聚集能力由修饰前的(1.43±0.74)%分别升至(3.86±1.21)%和(3.84±1.12)%;黏附能力由修饰前的(30.2±5.59)%分别升至(45.5±2.72)%和(45.2±3.99)%;而血小板Ca2+释放能力修饰前后差异无统计学意义。结论:使用mPEG-SPA对血小板进行化学修饰可影响血小板的功能,是一种较为理想的修饰剂。
Objective: To observe the functional changes of platelets modified by different mPEGs and to analyze their causes. Methods: The platelets were chemically modified with mPEG-BTC and mPEG-SPA respectively. The fluorescence intensity of platelet CD62P before and after modification was detected by flow cytometry. The aggregation, adhesion and release of platelets were detected. RESULTS: After modified with mPEG-BTC and mPEG-SPA, the expression of CD62P on platelets decreased from (12.77 ± 2.87)% to 0 and (4.12 ± 1.28)%, respectively. The aggregation ability was increased from 1.43 ± 0.74 before modification % Were increased to (3.86 ± 1.21)% and (3.84 ± 1.12)%, respectively. The adhesion capacity increased from (30.2 ± 5.59)% to (45.5 ± 2.72)% and Release ability modification before and after the difference was not statistically significant. CONCLUSION: The chemical modification of platelets by mPEG-SPA can affect platelet function and is an ideal modifier.