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从9个不同州市采取玉米茎基腐病病害样本分离纯化并鉴定其病原,分析云南省玉米茎基腐病的主要病原镰孢菌及分布情况,依据形态特征和rDNA-ITS序列分析,云南省玉米茎基腐病的病原包括Fusarium graminearum(40.74%)、F.verticillioides(37.96%)、F.fujikuroi(3.70%)、F.incarnatum(3.70%)、F.oxysporum(1.85%)、F.proliferatum(1.85%)、F.commune(5.55%)、F.chlamydosporum(2.78%)和F.redolens(1.85%)。F.graminearum和F.verticillioides为优势种,F.graminearum主要分布于昆明及其以东的地区,F.verticillioides主要分布于昆明市及其以西的地区,二者在云南的大部分地区均有分布;其余菌株出现频率均低于5.55%,仅分布在1~3个地区。
According to the morphological characteristics and rDNA-ITS sequence analysis, Yunnan province was divided into three parts: the main pathogen of Fusarium spp. The pathogens of corn-based rot disease in the province include Fusarium graminearum (40.74%), F. verticillioides (37.96%), F. fujikuroi (3.70%), F. incarnatum (3.70%), F. oxysporum (1.85%), proliferatum (1.85%), F.commune (5.55%), F. chlamydosporum (2.78%) and F. redolens (1.85%). F.graminearum and F.verticillioides are the dominant species, while F.graminearum is mainly distributed in Kunming and its east. F.verticillioides are mainly distributed in Kunming and its west, both of which are distributed in most parts of Yunnan The frequencies of other strains were less than 5.55%, only distributed in 1-3 areas.