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目的 :探讨急性单核细胞白血病原始细胞表达血小板特异性抗原的机制。方法 :分离外周血或骨髓单个核细胞 ,采用常规染色和普通细胞化学染色观察其形态学特性 ,采用流式细胞仪双荧光分析免疫表型 ,单荧光胞浆蛋白标记分析周期蛋白表达和免疫印迹进一步证实周期蛋白 D3的表达情况。采用周期蛋白和 DNA双标记分析周期蛋白 D3表达与细胞周期的关系。结果 :白血病原始细胞中 CD1 3 + + HL A- DR+细胞为 94.6 9% ,CD1 3+ + CD34+细胞为 96 .86 % ,CD41 a+ + CD34+ 细胞为 41.6 0 % ,CD1 3 + + CD41 a+ 细胞为 40 .0 0 % ,免疫印迹和流式细胞仪单参数分析证实周期蛋白 D3表达明显升高 ,周期蛋白 D3阳性细胞在细胞周期各时段所占的比例分别为 :G1 期 5 2 .10 % ,S期9.90 % ,G2 + M期 38.0 0 %。结论 :周期蛋白 D3的异常表达导致单核系白血病细胞表达血小板特异性抗原 ,同时也可能在白血病的发生中起重要作用。
Objective : To investigate the mechanism of expression of platelet-specific antigen in primitive cells of acute monocytic leukemia. METHODS: Peripheral blood or bone marrow mononuclear cells were isolated and their morphological characteristics were observed by routine staining and ordinary cytochemical staining. Immunophenotype was analyzed by flow cytometry dual-fluorescence, and cyclin expression was analyzed by single-fluorescent cytoplasmic protein labeling and western blotting. Further confirm the expression of cyclin D3. The relationship between cyclin D3 expression and cell cycle was analyzed using cyclin and DNA double-labeling. RESULTS: CD3+HL A-DR+ cells were 94.69% in CD4 3+ + CD34+ cells, 96.86% in CD1 3+ + CD34+ cells, and 41.6% in CD41a+ CD34+ cells. CD1 3 + CD41a+ cells were 40.0%, Western blot and flow cytometry analysis confirmed that the expression of cyclin D3 was significantly increased. The proportion of cyclin D3 positive cells in the cell cycle was 52.10% in G1 phase. S period 9.90 %, G2 + M period 38.0 0 %. CONCLUSION: The abnormal expression of cyclin D3 causes mononuclear leukemia cells to express platelet-specific antigens. It may also play an important role in the development of leukemia.