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目的:检测断乳期幼鼠胼胝体髓鞘蛋白PLP和MBP的表达,探讨丙烯酰胺(acrylamide,ACR)染毒对幼鼠胼胝体部髓鞘发育的影响。方法:断乳期幼鼠随机分为对照组(0 mg/kg)、低(18 mg/kg)和高(36 mg/kg)剂量组,每组12只,从出生后第22~42 d进行灌胃染毒。观测幼鼠步态的变化,用免疫组化方法和免疫荧光双标记法检测幼鼠胼胝体髓鞘蛋白脂蛋白(myelin PLP,PLP)和髓鞘碱性蛋白(myelin basic protein,MBP)的表达。结果:ACR染毒后幼鼠的步态评分均增加,差异有统计学意义(P<0.01),免疫组织化学检测结果显示,与对照组相比较,ACR高剂量组幼鼠大脑PLP和MBP表达减少,差异有统计学意义(P<0.05)。免疫荧光双标技术检测结果与免疫组织化学检测结果一致,即ACR染毒后胼胝体PLP和MBP均表达减少。结论:ACR染毒可能会通过减少PLP和MBP的表达,抑制胼胝体髓鞘的形成而影响神经系统发育。
Objective: To detect the expression of PLP and MBP in myelin sheath of young mice during weaning period and to investigate the effect of acrylamide (ACR) on the development of myelin sheath in young corpus callosum. Methods: Weaning young rats were randomly divided into control group (0 mg / kg), low (18 mg / kg) and high (36 mg / kg) Gavage infected. The changes of gait of the young rats were observed. The expressions of myelin PLP and myelin basic protein (MBP) were detected by immunohistochemistry and immunofluorescence double labeling. Results: Compared with the control group, the expression of PLP and MBP in the ACR high dose group was significantly higher than that in the control group (P <0.01). The results of immunohistochemistry showed that the expression of PLP and MBP Reduce, the difference was statistically significant (P <0.05). The immunofluorescence double-labeled technique was consistent with the results of immunohistochemistry, that is, the expression of PLP and MBP in the corpus callosum decreased after ACR exposure. CONCLUSION: ACR exposure may affect nervous system development by decreasing the expression of PLP and MBP, inhibiting the formation of corpus callosum myelin sheath.