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通过花蕾、子叶、子叶节、胚根和下胚轴培养,离体繁殖了‘绿洋’和‘绿岭’两个青菜花杂种。培养基为 MS+KT1+NAA0.1mg/l(下同)(花蕾);MS+KT5+IAA8+NAA0.01(子叶);B_5+KT6+IAA0.05+NaH_2PO_4·H_2O170(子叶节和下胚轴);B_5+KT3或4+IAA0.05+NaH_2PO_4·H_2O170,B_5+KT10+IAA0.05+NaH_2PO_4·H_2O170+LH500(下胚轴); MS+2,4-D_2+KT1,B_5+Z3.0+IAA1+LH500(胚根)。出枝率每个花蕾为3.0个;子叶为0.2个;子叶节和下胚轴为15～20个;在很少的情况下获得胚根再生植株。试管苗生根后移栽于蛭石中,成活率为74％(绿洋)和98％(绿岭)。定植于塑料大棚内,大部分生长发育正常,收获了无性系第一代花球。在显微切片上观察了各种外植体上新枝的形态发生过程。
The hybrids of ’Green Ocean’ and ’Green Ridge’ were cultivated in vitro through flower buds, cotyledons, cotyledonary node, radicle and hypocotyls. The medium was MS + KT1 + NAA0.1mg / l (same below) (flower bud); MS + KT5 + IAA8 + NAA0.01 (cotyledon); B5 + KT6 + IAA0.05 + NaH2PO4 · H20170 (cotyledonary node and lower embryo Axis); B_5 + KT3 or 4 + IAA0.05 + NaH2PO4 · H20170, B5 + KT10 + IAA0.05 + NaH2PO4 · H20170 + LH500 (hypocotyl); MS +2,4-D2 + KT1, B5 + Z3. 0 + IAA1 + LH500 (radicle). The budding rate was 3.0 for each flower bud; 0.2 for cotyledons; 15-20 for cotyledonary node and hypocotyls; radicle regenerated plants were obtained in rare cases. Test-tube seedlings were transplanted in vermiculite after rooting, with survival rates of 74% (green) and 98% (green ridge). Colonization in the plastic greenhouse, most of the normal growth and development, harvest the first generation of clonal bulbs. The morphology of new shoots on various explants was observed on the microsection.