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大量的研究表明,在器官缺血再灌注损伤所致肺损伤过程中,肿瘤坏死因子α(TNF α)和白细胞介素-1 β(IL-1 β) 都发挥了重要作用。本实验旨在观察肝缺血再灌注损伤过程中TNF α和IL-1 β在肺组织中表达的动态变化。一、材料与方法1.动物模型的制作与分组:选健康雄性Wistar大鼠48只(由新疆医科大学动物实验中心提供),体重200-250 g,随机分成6组,每组8只。第1组:假手术组;第2组:缺血30 min组(I组);第3组:缺血30 man再灌注组(I/R组);第4组:缺血30 min再灌注1 h组(I/R 1 h组);第5组:缺血30 min再
Numerous studies have shown that TNFα and IL-1β play an important role in the process of lung injury caused by organ ischemia-reperfusion injury. This experiment aimed to observe the dynamic changes of TNFα and IL-1β expression in the lung tissue during the process of hepatic ischemia-reperfusion injury. I. Materials and Methods 1. Animal Model Production and Grouping: 48 healthy male Wistar rats (provided by Animal Experimental Center, Xinjiang Medical University) weighing 200-250 g were randomly divided into 6 groups with 8 rats in each group. Group 1: Sham operation group; Group 2: Ischemia 30 min group (Group I); Group 3: Ischemia 30 man reperfusion group (I / R group); Group 4: Ischemia 30 min reperfusion 1 h group (I / R 1 h group); Group 5: ischemia 30 min again