Separation and Purification of GST-glycerol-3 -phosphate Dehydrogenase

• 作 者: Hongmei ZHAO Shihai LI Yasuo W
• 机 构: CollegeofAnimalSciences,AgricultureDepartment
• 出 处: 农业生物技术：英文版
• 发表日期: 2016年5期
• 关键词: 磷酸脱氢酶 酶的纯化 SDS-PAGE 重组酿酒酵母 NACL介质 GST融合蛋白 分离 诱导培养 Saccharomyces cerevisiae Glyc
• 基金项目: Supported by Social Service Project of New Countryside Development Research Institute of Yangtze University（201411 ）.

In order to investigate the expression of glycerol-3-phosphate dehydrogenase by GCY1 gene in recombinant Saccharomyces cerevisiae,induction culture of the S.cerevisiaestrain was performed with SD-URA 2% galactose,3 × YP + 6% glucose,SC-URA 2% galacto