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目的:研究广西甜茶总黄酮(RSTF)体外抗肿瘤作用。方法:广西甜茶总黄酮经60%的乙醇提取,聚酰胺柱分离后,用化学反应、薄层层析法及高效液相法进行鉴定;采用MTT法对肿瘤株S180(小鼠移植性肉瘤细胞)、H22(小鼠腹水型肝癌细胞)、L1210(小鼠白血病细胞)进行RSTF体外抗肿瘤作用筛选。结果:广西甜茶总黄酮在体外对肿瘤株S180,H22,L1210的增殖具有一定的抑制作用,其中对H22抑制作用最强(IC5046.31μg·mL-1);对S180抑制作用次之(IC5071.48μg·mL-1),对L1210抑制作用最弱(IC50163.59μg·mL-1)。结论:广西甜茶总黄酮在体外可抑制S180,H22和L1210肿瘤株的增殖。
Objective: To study the anti-tumor effect of total flavonoids from sweet tea (RSTF) in Guangxi. Methods: The total flavonoids of sweet tea from Guangxi were extracted with 60% ethanol and separated by polyamide column. The chemical constituents were identified by chemical reaction, TLC and HPLC. The tumor cell lines S180 (transplanted sarcoma cells ), H22 (mouse ascites hepatoma cells) and L1210 (mouse leukemia cells) were screened for anti-tumor effect in vitro by RSTF. Results: The total flavonoids of sweet tea from Guangxi had some inhibitory effects on the proliferation of S180, H22 and L1210 in vitro, of which the inhibitory effect on H22 was the strongest (IC5046.31μg · mL-1) and the inhibition on S180 was second (IC5071). 48μg · mL-1), the weakest inhibitory effect on L1210 (IC50163.59μg · mL-1). Conclusion: The total flavonoids of sweet tea can inhibit the proliferation of S180, H22 and L1210 tumor in vitro.