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By solution culture experiment, three wheat genotypes (Triticum aestivum L.) and two oilseed rape genotypes (Brassica napus L.) differing in Mn efficiency under Mn-deficient conditions were uaed to study mechanisms of the difference in Mn efficiency between wheat and oilseed rape. The results showed that there were significant differences in the abilities of MnIV reduction and acidification in root rhizosphere between the two species. Compared with wheat, oilseed rape had much higher reducing capacity and intensity of rhizosphere acidification under Mn-deficient conditions. Moreover, the higher ratio of functional leaves Mn/old leaves Mn in oilseed rape than in wheat was also an important factor for the different Mn efficiencies between the two species.