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在6MHz的压电晶振QCM(quartz crystal microbalance)金电极表面采用蛋白质A—金的方法固定生物敏感膜,利用QCM单面测试技术和连续流动自动分析系统全面测试分析此膜与人体免疫球蛋白G(IgG)之间的生物亲和反应,从而研究快速响应的压电晶体生物亲和传感器。停止流动法测量结果为:在1—2min内亲和响应随时间而急剧增大,随后趋于缓慢,对107μg/ml人体IgG三次响应的标准偏差及变异系数分别为2.5Hz和2.77％,在1—107μg/ml测量范围内响应值与测试浓度对数呈线性关系,用关系数和灵敏度分别为0.9824和53.6Hz/dec.(μg/ml):连续流动法测试结果为:进样时间约为1min,每个样品测试时间为3min,在2.9-249μg/ml测量范围内响应值与测试浓度对数呈线性关系,相关系数和灵敏度分别为0.9797和68.9Hz/dec.(μg/ml)。
The biosensitive membrane was immobilized on a 6MHz quartz crystal microbalance (QCM) gold electrode by protein A-gold method. The membrane was incubated with human immunoglobulin G using the QCM single-sided test technique and the continuous flow automated analysis system. (IgG) in order to study the rapid response of piezoelectric crystal bioaffinity sensors. The results of the stop-flow method were as follows: the affinity response rapidly increased with time within 1-2 min and then tended to be slow. The standard deviation and coefficient of variation of three-fold response to 107 μg / ml human IgG were 2.5 Hz and 2.77%, respectively. The response value in the range of 1-107μg / ml was linear with the logarithm of the test concentration. The correlation coefficient and sensitivity were 0.9824 and 53.6Hz / dec. (Μg / ml) respectively. The results of the continuous flow test were as follows: For 1 min, the test time for each sample was 3 min. The response was linear with the logarithm of the test concentration in the range of 2.9-249 μg / ml with a correlation coefficient and sensitivity of 0.9797 and 68.9 Hz / dec. (Μg / ml), respectively.