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目的探讨慢性乙肝患者前S1(Pre-S1)与HBeAg及HBV-DNA的相互关系。方法采用实时荧光定量聚合酶链反应技术(FQ-PCR)和酶联免疫吸附试验(ELISA)对931份乙肝不同阳性模式及35份乙肝阴性血清进行HBV-DNA及标志物检测。结果931份不同阳性模式乙肝患者中,HBeAg阳性总检出率为20.7%,Pre-S1抗原阳性总检出率为30.2%,HBV-DNA阳性总检出率为45.3%。在422例HBV-DNA阳性乙肝患者中,Pre-S1抗原阳性率为57.3%,HBeAg阳性率为44.1%,在281例Pre-S1阳性乙肝患者中,HBV-DNA阳性符合率86.1%,在Pre-S1阴性组HBV-DNA阳性率仅为27.7%(p<0.01)。结论HBV-DNA检测是反映HBV复制最直接的分子生物学指标,Pre-S1抗原比HBeAg更能灵敏反映HBV复制的血清学指标。
Objective To investigate the relationship between Pre-S1 and HBeAg and HBV-DNA in chronic hepatitis B patients. Methods HBV DNA and markers were detected by real-time fluorescence quantitative polymerase chain reaction (FQ-PCR) and enzyme-linked immunosorbent assay (ELISA) in 931 HBV positive and 35 HBV negative sera. Results Among 931 hepatitis B patients with different positive modes, the total positive rate of HBeAg was 20.7%, the positive rate of Pre-S1 antigen was 30.2%, and the positive rate of HBV-DNA was 45.3%. Among 422 HBV-DNA positive hepatitis B patients, the positive rate of Pre-S1 antigen was 57.3% and the positive rate of HBeAg was 44.1%. The positive coincidence rate of HBV-DNA in 281 pre-S1 positive hepatitis B patients was 86.1% The positive rate of HBV-DNA in -S1 negative group was only 27.7% (p <0.01). Conclusion HBV-DNA detection is the most direct molecular biological indicator of HBV replication. Pre-S1 antigen is more sensitive than HBeAg to serological markers of HBV replication.