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用人血浆组分IV中某些成分配制的低浓度牛血清（2％）培养液（称Ⅱ号培养液），培养Vero细胞的效果与美国GIBCO公司的低浓度牛血清培养基添加物Gms－A类似；用所培养的Vero细胞感染狂犬病毒，其病毒滴度均大于6．0logLD50／ml）；而残余牛血清含量从50～100μg／ml降至5～10μg／ml。
The effect of culturing Vero cells with low concentration bovine serum (2%) medium (referred to as culture medium No. 2) formulated with certain components of human plasma component IV is similar to that of Gms-A Similarly, rabies virus was infected with the cultured Vero cells with a titer greater than 6.0 logLD50 / ml), while the residual bovine serum level was reduced from 50-100 μg / ml to 5-10 μg / ml.