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为建立绒盖牛肝菌酸血药浓度和各主要组织的HPLC检测方法,考察绒盖牛肝菌酸在大鼠体内的药代动力学和组织分布特点。采用Agilent ZORBAX Eclipse XDB-C18 column色谱柱(4.6 mm×250 mm,0.5μm)分离,以V(甲醇)∶V(水)=75∶25为流动相,流速1.0 m L/min,柱温25℃,检测波长243 nm。研究结果表明大鼠血浆及肝组织中的内源性物质均不干扰样品的测定,线性关系良好,大鼠灌胃绒盖牛肝菌酸符合一室模型,主要药代动力学参数Tmax为(0.76±0.21)h,Cmax为(8.76±0.81)μg/m L,t1/2为(0.18±0.28)h,AUC0-inf为(102.95±0.78)μg/(m L·h),大鼠尾静脉注射绒盖牛肝菌酸符合二室模型,主要药代动力学参数t1/2为(0.33±0.71)h,AUC_(0-inf)为(34.14±2.38)μg/(m L·h)。该方法简单快速,准确可靠,符合生物样品的测定要求,并明确了绒盖牛肝菌酸在大鼠体内的药代动力学和组织分布特征。
In order to establish the HPLC method for the determination of the acid concentration in the bolete and the main tissues, the pharmacokinetics and tissue distribution of the porcini acid in the rat were investigated. The separation was performed on an Agilent ZORBAX Eclipse XDB-C18 column (4.6 mm × 250 mm, 0.5 μm) using a mobile phase of V (methanol): V (water) = 75:25 at a flow rate of 1.0 mL / ℃, detection wavelength of 243 nm. The results showed that the endogenous substances in the plasma and liver tissue of rats did not interfere with the determination of the sample, the linear relationship was good, the rat suede cap boletus acid meet the one-compartment model, the main pharmacokinetic parameters Tmax ( 0.76 ± 0.21 h, Cmax was (8.76 ± 0.81) μg / m L, t1 / 2 was 0.18 ± 0.28 h, AUC0-inf was (102.95 ± 0.78) μg / (m L · h) The main pharmacokinetic parameters t1 / 2 were (0.33 ± 0.71) h, AUC_ (0-inf) were (34.14 ± 2.38) μg / (m L · h) . The method is simple and rapid, accurate and reliable, which is in line with the determination requirements of biological samples and the pharmacokinetics and tissue distribution characteristics of the porcini acid in the rat body is clarified.