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本实验采用免疫组织化学ＡＢＣ－ＧＤＮ法和地高辛标记寡核苷酸探针原位杂交技术，研究了降钙素基因相关肽（ｃａｌｃｉｔｏｎｉｎｇｅｎｅ－ｒｅｌａｔｅｄｐｅｐｔｉｄｅ，ＣＧＲＰ）及其ｍＲＮＡ在恒河猴肺内的表达。结果显示ＣＧＲＰ分布于恒河猴肺内各级支气管粘膜上皮的神经内分泌细胞（ｎｅｕｒｏｅｎｄｏｃｒｉｎｅｃｅｌｓ，ＮＥＣ）和神经上皮样小体（ｎｅｕｒｏｅｐｉｔｈｅｌｉａｂｏｄｉｅｓ，ＮＥＢ）中。ＣＧＲＰ杂交阳性细胞的分布与免疫组织化学的结果相同。ＣＧＲＰｍＲＮＡ杂交信号均匀分布于整个细胞质，而ＣＧＲＰ免疫反应物仅在神经内分泌细胞的基部更明显，说明肽合成后聚集在基部。免疫组织化学还发现走行于肺内支气管和血管外周的神经纤维亦呈ＣＧＲＰ－ＩＲ阳性。相邻切片法还证实，ＣＧＲＰ与５－羟色胺（５－ＨＴ）共存于ＮＥＣ和ＮＥＢ中，说明二者在功能上可能具有相关性。
In this study, immunohistochemistry ABC-GDN method and digoxigenin labeled oligonucleotide probe in situ hybridization, calcitonin gene-related peptide (calcitoningene-related peptide (CGRP) and its mRNA in the rhesus monkey lung expression. The results showed that CGRP was distributed in neuroendocrinecels (NECs) and neuroepithelial bodies (NEBs) of bronchial mucosal epithelium in the lungs of rhesus monkeys. The distribution of CGRP positive hybrid cells is the same as that of immunohistochemistry. CGRP mRNA hybridization signal evenly distributed throughout the cytoplasm, and CGRP immunoreactive material only in neuroendocrine cells at the base of the more obvious, that the peptide aggregated in the base after the synthesis. Immunohistochemistry also found that walking in the lung bronchus and peripheral vascular fibers also showed CGRP-IR positive. Adjacent sections also confirmed that CGRP and 5-hydroxytryptamine (5-HT) co-exist in NEC and NEB, indicating that the two may be functionally related.