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目的:研究中药“松友饮”对高转移人肝癌裸鼠原位移植肿瘤生长、转移和生存期的影响。方法:以肝癌细胞(MHCC97H)细胞建立裸鼠移植瘤模型,32只成模裸鼠随机分成对照组C0和“松友饮”剂量递增的C1、C2和C3组,肝脏接种肿瘤24h内开始1次/d灌胃给药,连续5周,每周测体质量。第35d处死裸鼠,计算肿瘤体积及转移情况。另选12只成瘤裸鼠分成对照组和“松友饮”组,选择上述结果中最合适的剂量,观察生存期。结果:C0、C1、C2和C3组肿瘤体积分别为(2072.73±801.23)mm3、(1779.48±969.59)mm3、(976.30±608.01)mm3和(959.99±376.47)mm3,C2、C3与C0相比差别有统计学意义;C2组抑瘤率52.90%,肺转移程度明显下降。按C2组剂量给药,裸鼠生存期(75.00±3.86)d,对照组(52.00±2.34)d(P<0.01)。结论:连续5周“松友饮”灌胃抑制HCC裸鼠模型肿瘤生长、转移,延长荷瘤裸鼠生存期,对HCC病人有潜在的应用价值。
Objective: To study the effect of traditional Chinese medicine “Song Youyin” on the growth, metastasis and survival of orthotopic transplanted hepatocellular carcinoma in nude mice. Methods: Nude mice xenografts were established by hepatocellular carcinoma (MHCC97H) cells. Thirty-two nude mice were randomly divided into two groups: C0 group and C1 group, C2 group and C3 group. 1 / d gavage for 5 weeks, weekly body mass measurement. Nude mice were sacrificed on the 35th day to calculate the tumor volume and metastasis. Another 12 tumor-bearing nude mice were divided into control group and “Songyouyin” group, and the most appropriate dose was selected to observe the survival time. Results: The tumor volumes in C0, C1, C2 and C3 groups were (2072.73 ± 801.23) mm3, (1779.48 ± 969.59) mm3, (976.30 ± 608.01) mm3 and (959.99 ± 376.47) mm3, respectively There was statistical significance; C2 tumor inhibition rate was 52.90%, lung metastasis decreased significantly. According to the dosage of C2 group, the survival time of nude mice was (75.00 ± 3.86) d and that of control group (52.00 ± 2.34) d (P <0.01). CONCLUSION: Ginsenoside inhibits tumor growth and metastasis of nude mice in nude mice in 5 consecutive weeks and prolongs survival of nude mice bearing HCC. It has potential value for HCC patients.