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In this paper,the relationship between radiosensitivity,cell cycle alteration and thechange of apoptosis in different human hepatoma cell lines irradiated by heavy ions were studiedwith the aim of building up the base data for clinical therapy.Exponentially growing hepatomacell lines were irradiated by 80.55 MeV/u~(12)C~(6+) ions at a dose of 0 Gy,0.5 Gy,1 Gy,2 Gy,4 Gyand 8 Gy.The radiosensitivity was assessed by means of the colony-forming assay.The DNAcontent,the percentage of each cell-cycle phase and the apoptosis rate were obtained with flowcytometry methods.After the irradiation,the SF_2 (survival fraction at 2 gray) of SMMC-7721cells were evidently lower than that of HepG2 cells.The S phase arrest,G2/M phase arrest delayand the apoptosis in the two hepatoma cell lines varied with the increase of the dose and repairtime.The heavy ions could obviously kill the human hepatoma cell lines.Compared to HepG2cells.SMMC-7721 cells were more radiosensitive to ~(12)C~(6+) ions.
In this paper, the relationship between radiosensitivity, cell cycle alteration and the change of apoptosis in different human hepatoma cell lines irradiated by heavy ions were studied with the aim of building up the base data for clinical therapy. Exponentially growing hepatomacell lines were irradiated by 80.55 MeV / u ~ (12) C ~ (6+) ions at a dose of 0 Gy, 0.5 Gy, 1 Gy, 2 Gy, 4 Gy and 8 Gy.The radiosensitivity was assessed by means of the colony-forming assay.The DNA content, the percentage of each cell-cycle phase and the apoptosis rate were obtained with flowcytometry methods. After the irradiation, the SF_2 (survival fraction at 2 gray) of SMMC-7721 cells were evidently lower than that of HepG2 cells. The S phase arrest, G2 / M phase arrest delay and the apoptosis in the two hepatoma cell lines varied with the increase of the dose and repairtime. The heavy ions could obviously kill the human hepatoma cell lines. Compared to HepG2 cells. SMMC-7721 cells were more radiosensitive to ~ (12) C ~ (6+) ions.