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目的:通过对不同剂量的角叉菜胶诱导建立血瘀证树鼩模型表征及血液学观察,探索由角叉菜胶诱导的血瘀证树鼩模型的最佳剂量。方法:32只树鼩随机分为对照组、低剂量组(25 mg/kg)、中剂量组(50 mg/kg)、高剂量组(75 mg/kg),8只/组,腹腔注射角叉菜胶3 d,造模24 h后肉眼结合体征采集分析系统定性定量观察表征,全自动血流变快测仪检测血液流变学指标,全自动凝血分析仪检测凝血4项。结果:造模24 h后,造模动物精神萎靡、舌底脉络增粗增长,随着剂量增加,固定性疼痛概率增多、舌质、爪部和鼻唇部皮肤颜色变深变暗。血液流变学指标中,高剂量组全血黏度、红细胞压积、红细胞聚集指数、卡松屈服应力和血浆黏度明显升高(P<0.05或P<0.01),红细胞电泳时间显著延长(P<0.01),而红细胞变形指数降低(P<0.01);中、高剂量组的APTT、PT、TT明显降低(P<0.05或P<0.01),而FIB含量增加(P<0.01)。结论:高剂量的角叉菜胶所诱导的血瘀树鼩模型成立。
OBJECTIVE: To explore the optimal dosage of tree stalk model induced by carrageenan by establishing the tree stump model of blood stasis syndrome and hematological observation induced by different doses of carrageenan. Methods: Thirty-two tree shrews were randomly divided into control group, low dose group (25 mg / kg), middle dose group (50 mg / kg), high dose group (75 mg / Carrageenan 3 d, model 24 h after the naked eye combined with physical acquisition and analysis system qualitative and quantitative observation of characterization, automatic blood rheology detector to detect hemorheological indicators, automatic coagulation analyzer for the detection of coagulation 4. RESULTS: At 24 h after modeling, the model animals showed sluggishness and thickening of the venous veins. As the dose increased, the probability of fixed pain increased, and the color of the skin, tongue, claw and nasolabial folds became darker and darker. In hemorheology indexes, the whole blood viscosity, hematocrit, erythrocyte aggregation index, Casson’s yield stress and plasma viscosity were significantly increased (P <0.05 or P <0.01), and the erythrocyte electrophoresis time was significantly prolonged (P < 0.01), while the index of erythrocyte deformability decreased (P <0.01). The APTT, PT, TT of middle and high dose groups decreased significantly (P <0.05 or P <0.01), while FIB content increased (P <0.01). Conclusion: The model of blood stasis tree induced by high dose of carrageenan was established.