论文部分内容阅读
有些血清流行病学研究揭示疱疹病毒Ⅱ型(HSV—2)感染和宫颈癌之间存在着相关性。实验室内,经紫外线照射过的病毒可转化田鼠细胞,而转化细胞对田鼠有致癌性。曾报道,宫颈癌灶刮脱的细胞内有疱疹病毒抗原;应用核酸杂交技术,曾在一份宫颈癌中发现疱疹病毒DNA。然而,也有报道未能在活检材料中发现HSV抗原或DMA的。本文报道从宫颈癌建立细胞系的方法,并对这些细胞系的疱疹病毒标志进行检测。作者检查了29份侵润性宫颈癌和4份作为对照的正常宫颈上皮标本。剪碎组织块先经胶元酶处理,然后加入含10%胎牛血清的培养液进行培养。开始5—10天内生长的为类上皮细胞,但以后则为纤维母细胞所代替。自侵润性宫颈癌组织的培养物中,只有5份持
Some serum epidemiological studies have revealed a correlation between herpes simplex virus type 2 (HSV-2) infection and cervical cancer. In the laboratory, the virus that has been irradiated by ultraviolet light can transform the field mouse cells, while the transformed cells are carcinogenic to the field mice. It has been reported that cervical cancer cells scraping off herpes virus antigens; application of nucleic acid hybridization, was found in a case of cervical cancer herpes virus DNA. However, there are also reports of failure to detect HSV antigens or DMA in biopsy material. This article reports on the establishment of cell lines from cervical cancer and the detection of herpesviruses in these cell lines. The authors examined 29 invasive cervical cancers and 4 normal cervical epithelial specimens as controls. Scissor tissue first by collagenase treatment, and then added to the medium containing 10% fetal bovine serum culture. The first five days of growth of epithelial cells, but later replaced by fibroblasts. Of the cultures from invasive cervical cancer tissue, only 5 hold