产肠毒素大肠杆菌多重PCR检测方法的建立

来源 :中国预防兽医学报 | 被引量 : 0次 | 上传用户：super8516

【摘要】

：

为快速检测和鉴定产肠毒素大肠杆菌(ETEC)菌毛(K88和K99)和毒素(STa)基因,本研究设计合成了针对K88、K99和STa基因的3对特异性引物,对K88、K99和STa基因扩增条件进行优化,建

【作者】

：

曲泽慧陈佩佩张爱琴郭东春师东方曲连东

【机构】

：

东北农业大学动物医学院,黑龙江哈尔滨,150030黑龙江八一农垦大学动物科技学院,黑龙江大庆,163319;中国农业科学院哈尔滨兽医研究所,黑龙江哈尔滨,150001;

【出处】

：

中国预防兽医学报

【发表日期】

：

2012年12期

【关键词】

：

产肠毒素大肠杆菌多重PCR K88菌毛 K99菌毛 STa毒素

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为快速检测和鉴定产肠毒素大肠杆菌(ETEC)菌毛(K88和K99)和毒素(STa)基因,本研究设计合成了针对K88、K99和STa基因的3对特异性引物,对K88、K99和STa基因扩增条件进行优化,建立了检测K88、K99和STa的多重PCR方法.该方法对Kss、K99和STa基因的扩增产物分别为237 bp,314 bp和166 bp;此外,该方法具有良好的灵敏性和特异性.本实验建立的多重PCR方法为致幼畜腹泻ETEC的检测提供了快速准确方法.用所建立的多重PCR方法对实验室分离的23株大肠杆菌进行检测,结果2株为K99/STa阳性,1株为STa阳性.

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产肠毒素大肠杆菌多重PCR检测方法的建立

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