应用高速逆流色谱法分离茎点霉属真菌代谢物中脂肪酸成份的研究

• 作 者: 王志强 杨志钧 陈代杰 徐威
• 机 构: 上海来益生物药物研究开发中心,沈阳药科大学生命科学与生物制药学院,
• 出 处: 中国抗生素杂志
• 发表日期: 2010年06期
• 关键词: 高速逆流色谱 茎点霉属内生真菌 脂肪酸

利用高速逆流色谱对一株茎点霉属内生真菌的代谢物质脂肪酸部分进行了分离。采用的两相溶剂系统为正庚烷:乙酸乙酯:甲醇:水=8.5:1:8.5:1,上相做固定相,下相做流动相。分离得到三个脂肪酸组分A5、A6、A7,经高效液相分析,纯度都在99%以上,其回收率分别为96.3%、93.5%,94.8%。经EI-MS、1H-NMR测定,确定A5为硬脂酸,A6为油酸,A7为亚油酸。

High-speed countercurrent chromatography was used to separate the fatty acids from the metabolites of endophytic fungi of Phomopsis species. The two-phase solvent system used is n-heptane: ethyl acetate: methanol: water = 8.5: 1: 8.5: 1, the upper phase is the stationary phase and the lower phase is the mobile phase. Three fatty acid components, A5, A6 and A7, were separated and analyzed by HPLC. The purity of the three fatty acids was over 99%. The recoveries were 96.3%, 93.5% and 94.8%, respectively. The result of EI-MS and 1H-NMR showed that A5 was stearic acid, A6 was oleic acid, and A7 was linoleic acid.