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通过综合评价品种(系)的耐寒性,筛选出耐寒性较好的品种。以云南农业大学自育的9个农艺性状优异的甘蔗品系为研究对象,在甘蔗苗期进行低温处理,通过抗逆生理生化指标的变化,采用极点排序法对各品种(系)进行综合评价。结果表明:1低温(3℃)处理使甘蔗叶片的细胞膜电导率、可溶性糖含量、丙二醛含量、脯氨酸含量、可溶性蛋白含量、叶绿素含量、过氧化物酶(POD)活性分别平均增加了21.21%、134.1%、83.60%、35.47%、47.72%、9.07%、565.2%。2低温(3℃)处理使甘蔗叶片的超氧化物歧化酶(SOD)活性平均下降19.67%。最终筛选出耐寒性最好的甘蔗品种‘滇蔗03-91’,耐寒性最差的甘蔗品种‘滇蔗02-39’,而‘滇蔗08-5’、‘滇蔗05-103’、‘滇蔗01-58’等品种(系)耐寒性强于‘滇蔗04-14’、‘滇蔗04-429’、‘滇蔗05-522’、‘滇蔗02-227’品种(系)。
By comprehensively evaluating the cold tolerance of varieties (lines), the varieties with better cold resistance were selected. Nine sugarcane lines with excellent agronomic traits self-developed by Yunnan Agricultural University were selected as the research objects. The sugarcane lines were treated at low temperature in the seedling stage of sugarcane, and the varieties (lines) were evaluated comprehensively by the method of pole-rank sequencing. The results showed that: (1) Low temperature (3 ℃) increased the cell membrane conductivity, soluble sugar content, malondialdehyde content, proline content, soluble protein content, chlorophyll content and peroxidase (POD) 21.21%, 134.1%, 83.60%, 35.47%, 47.72%, 9.07%, 565.2%. 2 Low temperature (3 ℃) treatment decreased the superoxide dismutase (SOD) activity of sugarcane leaves by 19.67% on average. Finally, the sugarcane variety ’Yunnan Cane 03-91’ with the best cold tolerance was screened out, the sugarcane variety ’Yunnan Cane 02-39’ with the worst cold tolerance was selected, and the sugarcane 08-5 ’, Yunnan Cane 05-103’ The cold tolerance of ’Yunnan sugarcane 01-58’ and other varieties (lines) was stronger than that of ’Yunnan Dianxian 04-14’, ’Yunnan Dianxian 04-429’, ’Yunnan Dianxian 05-522’ and ’Yunnan Dianxian 02-227’ ).