论文部分内容阅读
目的：为探讨Ｐ１６基因的改变与膀胱移行细胞癌生物学行为的关系。方法：采用聚合酶链反应－单链构象多态性分析（ＲＣＲ－ＳＳＣＰ），对４０例膀胱移行细胞癌组织Ｐ１６基因纯合缺失、突变进行了研究。结果：膀胱移行细胞癌中Ｐ１６纯合缺失与突变率在Ⅰ、Ⅱ、Ⅲ级肿癌中分别为７．１％、１８．８％、６０％。在Ｔｉｓ～Ｔ_１期和Ｔ_２～Ｔ_４期分别为５．６％及４０．９％且与肿瘤细胞的分化程度、临床分期明显相关。结论：Ｐ１６基因异常可能在膀胱移行细胞癌的发生发展中起作用。
Objective: To investigate the relationship between the alteration of P16 gene and the biological behavior of bladder transitional cell carcinoma. METHODS: Polymerase chain reaction-single strand conformation polymorphism analysis (RCR-SSCP) was used to study the homozygous deletion and mutation of P16 gene in 40 bladder transitional cell carcinomas. Results: The homozygous deletion rate and mutation rate of P16 in bladder transitional cell carcinoma were 7.1%, 18.8%, and 60% in grade I, II, and III tumors, respectively. It was 5.6% and 40.9% in Tis-T1 phase and T2-T4 phase, respectively, and was significantly related to the differentiation and clinical stage of tumor cells. Conclusion: P16 gene abnormalities may play a role in the development of bladder transitional cell carcinoma.