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为了解骨形态发生蛋白（ＢＭＰ）在关节软骨损伤与修复及异位化骨中的作用，本研究观察了部分纯化的牛骨形态发生蛋白（ｂＢＭＰ）和高度纯化的人骨形态发生蛋白（ｈＢＭＰ）对体外贴壁培养原代、反分化人胎儿关节软骨细胞及鼠３Ｔ３成纤维细胞ＤＮＡ、胶原和蛋白多糖合成的影响。结果发现：部分纯化的ｂＢＭＰ和高度纯化的ｈＢＭＰ均不促进原代人关节软骨细胞合成ＤＮＡ和胶原，并显著抑制其蛋白多糖的合成。部分纯化的ｂＢＭＰ对反分化关节软骨细胞和鼠３Ｔ３成纤维细胞的ＤＮＡ合成、胶原合成和蛋白多糖合成却有明显促进作用。因此，本研究认为，ＢＭＰ诱导下的关节软骨细胞不会发生反分化，而有可能进一步向肥大软骨细胞样细胞或成骨细胞样细胞方向分化，并可能使反分化的软骨细胞重新表达软骨细胞表型。
In order to understand the role of bone morphogenetic protein (BMP) in the injury and repair of articular cartilage and ectopic bone, partially purified bBMP and highly purified human bone morphogenetic protein (hBMP) On Adherent Cultured Primary, Dedifferentiated Human Fetal Articular Chondrocytes and 3T3 Fibroblasts DNA, Collagen and Proteoglycan Synthesis. The results showed that both partially purified bBMP and highly purified hBMP did not promote the synthesis of DNA and collagen in primary human articular chondrocytes and significantly inhibited their proteoglycan synthesis. Partially purified bBMP significantly promoted the DNA synthesis, collagen synthesis and proteoglycan synthesis of differentiated articular chondrocytes and murine 3T3 fibroblasts. Therefore, the present study suggests that BMP-induced articular chondrocytes do not undergo anti-differentiation and may further differentiate into hypertrophic chondrocyte-like cells or osteoblast-like cells and may cause the re-differentiated chondrocytes to re-express chondrocytes Phenotype.