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利用根瘤土壤杆菌Ｔｉ质粒转化系统，采用叶盘法将ＣＭＶ－Ｃｐ基因转入番茄细胞中，获得４２株转基因植株，经Ｓｏｕｔｈｅｒｎｂｌｏｔ和Ｗｅｓｔｅｒｎｂｌｏｔ检测，证明ＣＭＶ－ｃｐ基因已进入番茄核染色体中，并能正常表达。通过对转基因植株Ｒ１和Ｒ２代苗期人工接种ＣＭＶ鉴定，发现ＣＭＶ－Ｃｐ基因产物对ＣＭＶ有一定抗性，其抗性遗传符合孟德尔一对基因控制性状的遗传规律并能稳定遗传。
Using the Ti plasmid transformation system of Agrobacterium tumefaciens, CMV-Cp gene was transferred into tomato cells by leaf disc method, and 42 transgenic plants were obtained. The result of Southern blot and Western blot showed that CMV-cp gene has entered the chromosome of tomato, Normal expression. CMV-Cp gene products were found to have some resistance to CMV by identifying CMV of transgenic plants R1 and R2 at the seedling stage. The inheritance of CMV-Cp gene was consistent with that of Mendel’s genetic control trait and could be inherited stably.