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作者曾利用金仓鼠肺恶性转化细胞系(BHL B_4)进行过一系列的癌变原理研究。为进一步利用该系统研究人抗癌基因在BHL B_4细胞中的表达,以至利用该系统进行抗癌基因的分离,本组用细胞融合技术建立了人鼠杂种细胞系,并对其进行了初步鉴定。 实验方法:处于对数生长期的BHL B_4细胞用MNNG诱发突变,经过一段突变表达期后用6-TG和
The authors have conducted a series of studies on the principle of canceration using the golden hamster lung malignant transformation cell line (BHL B_4). To further use this system to study the expression of human anti-oncogenes in BHL B_4 cells and even use this system to isolate anti-oncogenes, this group established a human and murine hybrid cell line using cell fusion technology and conducted preliminary identification. . Experimental method: MNNG-induced mutations in BHL B_4 cells in logarithmic growth phase, 6-TG after a mutated expression period