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研究了曙红Y(EY)的共振散射光谱、荧光光谱和吸收光谱,讨论了共振光散射与共振荧光的区别与联系。在EY水溶液三维荧光等高线光谱图中,瑞利散射线与荧光等高线有部分相交。EY的共振散射峰(5 2 5nm)介于荧光激发峰(5 1 4nm)和发射峰(5 3 6nm)之间。由光偏振实验,测得EY共振散射光谱5 2 5nm处的偏振度P =0 2 0。上述实验结果证明,EY的共振散射峰主要是共振荧光。在改变pH的实验中发现,EY共振光散射增强是由于酸碱平衡的移动导致荧光型体的形成。由于自吸收的影响,共振散射光强度与EY浓度之间不是严格的线性关系。
The resonance scattering spectra, fluorescence spectra and absorption spectra of eosin Y (EY) were studied. The differences and relations between resonance light scattering and resonance fluorescence were discussed. In the EY aqueous solution of three-dimensional fluorescence contour map, Rayleigh scattering and fluorescence contour lines partially intersect. Resonance scattering peak (525nm) of EY was between fluorescence excitation peak (514nm) and emission peak (536nm). From the optical polarization experiment, the degree of polarization P = 0 2 0 at 525 nm of the EY resonance scattering spectrum was measured. The experimental results show that, EY resonant resonance peak is mainly resonant fluorescence. In the experiment of changing the pH, it was found that the enhancement of EY resonance light scattering is due to the formation of a fluorescent body due to the shift of acid-base balance. Due to the effect of self-absorption, there is not a strict linear relationship between the intensity of the resonant scattered light and the concentration of EY.