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以多聚核苷酸Oligo-p Ta535-1和Oligo-p Sc119.2-1为探针对济麦系列小麦进行双色荧光原位杂交(FISH)分析,结果发现,Oligo-p Ta535-1的FISH信号主要分布在A和D染色体上,而Oligop Sc119.2-1的FISH信号主要分布在B染色体上;济麦系列小麦的FISH位点较小麦中国春差异位点主要集中在染色体4A、6B、7B和1D染色体上,且多为Oligo-p Sc119.2-1信号;济南17的1B染色体着丝粒区Oligo-p Ta535-1信号、济麦20的1D短臂末端和2A长臂末端的Oligo-p Sc119.2-1信号、济麦262的5A长臂中间的Oligo-p Sc119.2-1信号可分别作为细胞学标签用于其身份识别。济麦系列小麦标准FISH核型的建立为利用染色体工程对其进行改良奠定了良好的细胞遗传学基础;FISH信号变异位点的类似性,说明济麦系列小麦的遗传基础相对狭窄,应在今后育种工作中注意选择与其亲缘关系相对较远的育种亲本进行组配。
Two-color fluorescence in situ hybridization (FISH) analysis of Jimai wheat with Oligo-p Ta535-1 and Oligo-p Sc119.2-1 as probes revealed that Oligo-p Ta535-1 The FISH signal was mainly distributed on chromosomes A and D, while the FISH signal of Oligop Sc119.2-1 mainly distributed on chromosome B. The FISH loci of wheat in Jimai series were mainly located on chromosomes 4A and 6B , 7B and 1D chromosomes, and most of them were Oligo-p Sc119.2-1 signal; Oligo-p Ta535-1 signal of chromosome 1B of chromosome 17 of Jinan 17, the 1D short arm end and the long arm distal end of 2A of Jimai 20 Of Oligo-p Sc119.2-1 signal, and the Oligo-p Sc119.2-1 signal in the middle of 5A long arm of Jimai 262 can be used as a cytological tag for their identification respectively. The establishment of the standard FISH karyotype of Jima series wheat laid a good cytogenetics basis for the improvement by chromosome engineering. The similarity of FISH signal variation loci indicates that the genetic basis of Jimai series wheat is relatively narrow and should be in the future Breeding work to pay attention to choose their genetic relationship is relatively far from the parents of breeding.