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采用形态鉴定,碳、氮源利用及耐药性试验对生防真菌F1的分类地位和生物学特性进行了研究,并利用盆栽、温室生物实验及离体实验研究了F1对辣椒疫病的防效及防病机理。结果表明:(1)F1为曲霉属[A s-p erg illusM iche li ex F r]舒展曲霉(A.effusus T irabosch i);(2)F1对辣椒疫病防效明显,在盆栽线椒和温室甜椒生防实验中的相对防效分别为83.3%和94.1%;(3)F1对辣椒疫霉菌丝生长相对抑菌率为57%~100%,对游动孢子萌发相对抑制率为78.9%;(4)F1能在辣椒根内定殖,单独接种F1与F1+P3混合接种后8,24和42 d时,F1的定殖密度分别为4×103/g,5×103/g,3×103/g和13×103/g,3.5×103/g,2.3×103/g;(5)接种F1对辣椒叶片苯丙氨酸解氨酶(PAL)与多酚氧化酶(PPO)活性有影响。接种24 d时,单独接种F1处理辣椒叶片PAL与PPO酶活分别较不接菌对照降低47.9%与26.3%,而F1+P3混合接种处理辣椒叶片PAL活性较对照提高86.7%,PPO活性较对照降低29.9%。
The taxonomic status and biological characteristics of biocontrol fungi F1 were studied by morphological identification, carbon and nitrogen source use and drug resistance test, and the effects of F1 on pepper blight were studied by means of pot experiment, greenhouse biological experiment and in vitro experiment And disease prevention mechanism. The results showed as follows: (1) F1 was A. stearothermophilus (A.effusus T irabosch i); (2) F1 had obvious control effect on pepper blight, The relative control efficiencies in pepper biocontrol experiment were 83.3% and 94.1% respectively. (3) The relative inhibitory rate of F1 to Phytophthora capsici mycelial growth was 57% -100% and that of zoospore germination was 78.9%. (4) F1 could colonize in pepper roots. The colonization density of F1 was 4 × 103 / g, 5 × 103 / g, 3 × 10 4, respectively at 8, 24 and 42 d after inoculation of F1 and F1 + P3 alone (5) The activity of phenylalanine ammonia lyase (PAL) and polyphenol oxidase (PPO) in pepper leaves were inoculated with F1 at the concentrations of 10 / g and 13 × 103 / g, influences. On the 24th day after inoculation, the activity of PAL and PPO decreased by 47.9% and 26.3% compared with the non-inoculated control, respectively. However, the activity of PAL increased by 86.7% Reduce by 29.9%.