论文部分内容阅读
作为化学生物学研究的重要方向,生物正交反应的发展与应用为生命科学研究提供了有力武器.利用生物正交反应,人们可以将合成分子与目标天然大分子在特定位点上实现特异性连接,进而达成诸如标记、定位、功能化、固定等一系列目标.生物模拟转氨反应及其衍生反应是一类特异性蛋白质N端修饰的生物正交反应,与天然蛋白侧链、C端的连接反应相互补充.由于该反应具有高效性、通用性、温和性及不需要引入突变或非天然氨基酸等优点,从发现以来已较为广泛地运用于蛋白质化学生物学的多个相关领域.在介绍其基本原理及反应优化的基础上,综述该类反应的发展及应用情况.
As an important direction of chemical biology, the development and application of bio-orthogonal reaction provides a powerful weapon for life science research.Using bio-orthogonal reaction, one can realize the specificity of synthetic molecules and target natural macromolecules at specific sites To achieve a series of goals such as labeling, localization, functionalization, immobilization, etc. Bioimpedance transamination reaction and its derivatization reaction are a kind of biological orthogonal reaction of N-terminal modification of specific protein, Since the reaction has the advantages of high efficiency, versatility, mildness and the need to introduce mutations or non-natural amino acids, it has been widely used in many related fields of protein chemical biology since its discovery. The basic principle and reaction optimization based on the review of the development of such reactions and applications.