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采用脾种植法复制裸鼠肝癌模型 ,待 6 0d转移灶形成后分别取肝、肾、肠系膜淋巴结等 ,以常规方法制成组织切片 ,ABC免疫组化染色 ,同时用人肝癌细胞株 (HHCC)作对照。结果 ,试验组 30例标本中 β 葡萄糖苷酸酶 (βG)表达阳性率达 10 0 % ,PCNA指数阳性率为 96 .7% ,nm 2 3表达阳性率高达 13 .3 % ;而对照组 30例标本中 βG表达阳性率为3 .3 % ;PC NA指数阳性率为 33 .6 % ;nm 2 3表达阳性率高达 10 0 %。试验数据经统计学处理 ,具有显著性差异 (P <0 .0 1)。表明 βG改变PCNA、nm 2 3等肿瘤相关因素在体内的表达是肝癌细胞转移能力增强的原因之一。
The spleen implantation method was used to replicate the nude mice liver cancer model. After liver metastases were established on the 60th day, liver, kidney and mesenteric lymph nodes were taken respectively. Tissue sections were made by conventional methods, ABC immunohistochemical staining was performed, and human hepatoma cell lines (HHCC) were used. Control. As a result, the positive rate of β-glucuronidase (βG) expression in 30 specimens of the experimental group was 100%, the positive rate of PCNA index was 96.7%, and the positive rate of nm 2 3 expression was as high as 13.3%; while the control group 30 The positive rate of βG expression in the specimens was 3.3%; the positive rate of PCNA index was 33.6%; the positive rate of nm23 expression was as high as 100%. The test data were statistically processed with significant differences (P < 0.01). It has been shown that βG changes the expression of PCNA, nm23, and other tumor-related factors in vivo, which is one of the reasons for the enhanced metastatic capacity of hepatoma cells.