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目的探讨缺氧标记物碳酸酐酶IX(CA-IX)在直肠癌患者不同部位表达和对直肠癌细胞体外生长的影响,以及在直肠癌发生和转移中的意义。方法应用Western blot方法检测CA-IX在正常直肠组织,直肠腺瘤,直肠癌原发癌灶,系膜组织,肝转移组织中的表达;MTT法检测CA抑制剂乙酰唑胺对体外直肠癌细胞生长能力的影响,判断CA-IX作为直肠癌相关蛋白和转移相关因子的意义。结果直肠癌癌灶CA-IX阳性率(74.2%)明显高于直肠腺瘤(16.7%)(P<0.01),而正常直肠组织无CA-IX表达。癌灶和系膜组织中CA-IX阳性表达与患者性别、肿瘤大小、分化程度、临床分期无关(均P>0.05)。N0组癌灶CA-IX阳性率(85.5%)高于N1组(66.7%)和N2组(64.5%)(均P<0.05);N0组系膜组织CA-IX阳性率(5.5%)低于N1组(26.2%)和N2组(29.0%)(均P<0.05)。乙酰唑胺能提高不同浓度5-氟尿嘧啶体外对直肠癌细胞杀伤能力(P<0.05)。结论 CA-IX能促进直肠癌细胞增殖,与直肠癌发生、发展有关,可作为直肠癌相关肿瘤标记物,但不宜作为癌转移相关因子。
Objective To investigate the effect of hypoxia marker carbonic anhydrase IX (CA-IX) on the expression of different sites in rectal cancer patients and on the growth of rectal cancer cells in vitro and its significance in the carcinogenesis and metastasis of rectal cancer. Methods Western blot was used to detect the expression of CA-IX in normal rectum, rectum adenocarcinoma, primary rectal cancer foci, mesangial tissue and liver metastasis. MTT assay was used to detect the inhibitory effect of CA inhibitor acetazolamide on rectal cancer cells Growth capacity, to determine the significance of CA-IX as a rectal cancer-related protein and metastasis-related factors. Results The positive rate of CA-IX in rectal cancer (74.2%) was significantly higher than that in rectal adenomas (16.7%) (P <0.01), while no expression was found in normal rectal tissues. The positive expression of CA-IX in cancer and mesangial tissues was not related to the gender, tumor size, differentiation and clinical stage (all P> 0.05). The positive rate of CA-IX in the N0 group was significantly higher than that in the N1 group (66.7%) and N2 group (64.5%) (all P <0.05). The positive rate of CA-IX in the N0 group was lower In N1 group (26.2%) and N2 group (29.0%) (all P <0.05). Acetazolamide increased the cytotoxicity of 5-Fluorouracil to rectal cancer cells in vitro (P <0.05). Conclusion CA-IX can promote the proliferation of rectal cancer cells, which is related to the occurrence and development of rectal cancer. It can be used as a tumor marker related to rectal cancer, but not as a relevant factor of cancer metastasis.