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2003,61(5-6):441] 在含有Hank’s盐、非必需氨基酸及5％胎儿小牛血清的MEM(minimum essential medium)培养基中,使用灌流培养模式且微载体浓度为4g Cytodex 3/L时,最高的细胞浓度可达4×10~6细胞/ml。用生长在上述优化条件下的BHK-212细胞研究狂犬病毒的生产(PV/BHK-21菌株)。在补充0.2％牛血清白
2003, 61 (5-6): 441] In a minimal essential medium (MEM) medium containing Hank’s salts, nonessential amino acids and 5% fetal bovine serum using a perfusion culture mode with a microcarrier concentration of 4 g Cytodex 3 / L, the highest cell concentration up to 4 × 10 ~ 6 cells / ml. Rabies virus production (PV / BHK-21 strain) was studied with BHK-212 cells grown under the optimized conditions described above. In supplemented with 0.2% bovine serum