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目的 探索应用树突状细胞肿瘤疫苗治疗肺癌血源性转移的可能性。方法 将Lewis肺癌细胞经尾静脉注射给C57BL/6J纯系小鼠建立肺癌血源性转移模型,应用树突状细胞负载合成的抗原多肽MUT-1,作为肿瘤疫苗进行免疫治疗。存活的小鼠再用10 倍数量的癌细胞皮下攻击,观察其免疫保护效应。这些小鼠的脾脏T淋巴细胞经纯化后进行体外抗原致敏,然后测定其特异性细胞毒效应。结果 尾静脉注射Lew is肺癌细胞可引起多脏器肿瘤播散,造成所有荷瘤小鼠死亡。但在注射Lewis肺癌细胞后24 h,用树突状细胞疫苗进行免疫治疗,可以完全控制转移病灶的形成及因肿瘤转移引起的死亡,这些小鼠对10 倍数量的Lew is 肺癌细胞再次攻击,具有显著的免疫保护作用。其脾脏T淋巴细胞对Lewis肺癌细胞具有特异性细胞毒效应。结论 树突状细胞疫苗治疗能够有效地清除血源性播散的肺癌细胞,其抗癌机理是形成强有力的特异性细胞免疫应答
Objective To explore the possibility of using dendritic cell tumor vaccine to treat hematogenous metastases from lung cancer. Methods Lewis lung cancer cells were injected into C57BL/6J pure mouse via tail vein to establish a hematogenous metastasis model of lung cancer. The antigen peptide MUT-1 loaded with dendritic cells was used as tumor vaccine for immunotherapy. The surviving mice were subcutaneously challenged with 10 times the number of cancer cells, and their immunoprotective effects were observed. The spleen T lymphocytes of these mice were purified and then sensitized in vitro and their specific cytotoxic effects were measured. Results The tail vein injection of Lew is lung cancer cells can cause the spread of multiple organ tumors, resulting in the death of all tumor-bearing mice. However, 24 hours after the injection of Lewis lung cancer cells, immunotherapy with dendritic cell vaccines can completely control the formation of metastatic lesions and death due to tumor metastases. These mice are again attacking 10 times the number of Lew is lung cancer cells. Has a significant immune protection. The spleen T lymphocytes have specific cytotoxic effects on Lewis lung cancer cells. Conclusion The treatment of dendritic cell vaccine can effectively eliminate hematogenous disseminated lung cancer cells. The anti-cancer mechanism is to form a powerful specific cellular immune response.