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The objective of this research was to improve the growth and biomass of Pterocarpus santalinus L.f.(an endangered leguminous tree) using native microbial sym-bionts such as arbuscular mycorrhizal fungi and Rhizobium associated with native populations of P.santalinus.The native arbuscular mycorrhizal fungi isolated from P.santa-linus soils were identified as (1) Glomus fasciculatum;(2)Glomus geosporum;and Glomus aggregatum.A nitrogen-fixing microbial symbiont was isolated from the root nodules of P.santalinus and identified as Rhizobium aegyptiacum by 16s rRNA gene sequencing.These microbial symbionts were inoculated individually and in combination into P.santali-nus seedling roots.After 90 days,growth and biomass had improved compared with uninoculated controls.Shoot and root lengths,number of leaves,stem circumference,number of root nodules,biomass,nutrient uptake and seedling qual-ity index were significantly increased by a combined inocu-lation of arbuscular mycorrhizal fungi+Rhizobium aegyp-tiacum.It was concluded that native microbial symbionts positively influenced P.santalinus seedling growth which will be helpful for successful field establishment.