论文部分内容阅读
目的:对熏烟叠加气管内滴注脂多糖(LPS)所致慢性阻塞性肺病(COPD)急性期大鼠模型进行中医基本证候的判定。方法:通过动态采集模型动物呼吸、活动、皮毛、二便等宏观体征;运用病理形态学观察模型动物肺及支气管组织的微观变化。以临床证候诊断标准为依据,根据动物宏观体征、病理学变化等信息,通过与临床COPD各证候诊断标准进行同质性比较与鉴别,进而对动物模型进行证候属性的判定。结果:模型动物出现呼吸喘促,咳嗽,口鼻气管内分泌物明显增多,大便干结,小便颜色由淡黄色变为亮黄色,被毛灰黄无光泽,头部毛发脱落稀疏,饮食明显减少,体重增长明显减慢,舌体及爪甲颜色均较对照组深。结论:通过致病之因、模型动物的证候体征特点以及与临床各证型的比较与鉴别可知采用熏烟叠加气管内滴注LPS复合法构建COPD急性期模型与临床上COPD“痰热壅肺证”证候特点最为接近。
OBJECTIVE: To determine the basic TCM syndromes of acute exacerbation of chronic obstructive pulmonary disease (COPD) rat model by intratracheal intratracheal instillation of cigarette smoke. Methods: Macroscopic signs of the animals’ breathing, activity, fur, cats and the like were dynamically collected. The microscopic changes of lung and bronchial tissues of the model animals were observed by pathological morphology. Based on the diagnostic criteria of clinical syndromes, according to the macroscopic signs and pathological changes of animals, the homogeneity was compared with the diagnostic criteria of clinical syndromes of COPD, and then the syndrome property of animal models was judged. Results: The model animals appeared wheezing and coughing, respiratory and tracheal secretions increased obviously, stool became dry, urine color changed from light yellow to bright yellow, hair gray and yellow dull, sparse hair loss on the head, the diet was significantly reduced, body weight Growth slowed down significantly, tongue and claw Jia color than the control group. Conclusion: Through the pathogenic causes, the characteristics of the syndromes of the model animals and the comparison with the clinical syndromes, we can see that the COPD acute phase model and the clinical COPD “sputum heat壅 lung card ”Syndrome characteristics are the closest.