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目的探讨牛初乳对感染轮状病毒乳鼠的保护作用。方法检测粪便中轮状病毒的抗原以及建立轮状病毒感染乳鼠模型,分别给予牛初乳高剂量(50g/L)、中剂量(25g/L)、低剂量(10g/L)和利巴韦林(3g/L)治疗6天,每天观察乳鼠的食欲、体重、腹泻率、腹泻严重程度及活动,同时观察小肠组织病变情况。结果乳鼠感染轮状病毒12h后均出现不同程度腹泻。模型组在第3-4天腹泻最严重,腹泻评分为3.5,且体重一直处于低水平,与正常组相比明显减轻(P<0.01);牛初乳高剂量组与利巴韦林组在第3天腹泻最严重,但腹泻分数最高2.5,低于模型组,差异显著(P<0.05),两组体重增加与模型组相比有显著差异(P<0.05),与正常组无统计学差异(P>0.05)。病理结果显示,正常对照组与牛初乳高剂量组小肠黏膜无明显肿胀、充血,黏膜下少量淋巴细胞浸润,绒毛清楚;模型组的小肠黏膜变薄,黏膜肿胀、充血,大量淋巴细胞浸润,绒毛脱落。结论牛初乳高剂量组对肠道感染轮状病毒乳鼠模型有减轻腹泻、降低腹泻率,对小肠黏膜具有修复作用。
Objective To investigate the protective effect of bovine colostrum on rotavirus infected neonatal rats. Methods To detect the rotavirus antigen in the stool and to establish a rotavirus infected neonatal rat model. The bovine colostrums were treated with high dose (50g / L), medium dose (25g / L), low dose (10g / L) Waring (3g / L) for 6 days, daily observation of the appetite, body weight, diarrhea rate, diarrhea severity and activity of the suckling mice, and observe the intestinal tissue lesions. Results The mice were infected with rotavirus 12h after varying degrees of diarrhea. The model group had the most severe diarrhea on day 3-4, the diarrhea score was 3.5, and the body weight was always at a low level, which was significantly lower than that of the normal group (P <0.01). In the high dose of bovine colostrum group and ribavirin group, The diarrhea was most serious on the third day, but the highest diarrhea score was 2.5, lower than the model group, the difference was significant (P <0.05). There was significant difference between the two groups (P <0.05) Difference (P> 0.05). The pathological results showed that there was no obvious swelling, congestion and a few lymphocytes infiltration in mucous membrane in the normal control group and bovine colostrum high dose group, and the villi were clear. The intestinal mucosa of the model group was thinner, the mucosa was swollen, hyperemia, infiltration of lymphocytes, Villus shedding. Conclusion High-dose bovine colostrums can reduce diarrhea, reduce diarrhea rate and repair intestinal mucosa in rotavirus model of intestine infection.