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以感染松材线虫病的马尾松林土壤作为研究对象,探索不同程度松材线虫病感染对马尾松林土壤理化性质、微生物生物量和土壤酶活性的影响。结果表明:随松材线虫病危害程度的加重,总碳、总磷、总钾、可溶性有机碳、铵态氮、硝态氮、有效磷和含水量呈现升高趋势,而p H、Ca、Mg、可溶性有机氮、微生物生物量碳(MBC)和微生物生物量氮(MBN)显著降低;同时,蔗糖酶、脲酶、纤维素酶和多酚氧化酶酶活性随着感染程度的加重而趋于下降,而酸性磷酸酶和蛋白酶活性则显著升高。结果表明:土壤脲酶、蔗糖酶、纤维素酶和多酚氧化酶4种酶活性与含水量、铵态氮、硝态氮、可溶性有机碳、总磷、总钾、有效磷、总碳等理化指标呈显著负相关,而与可溶性有机氮、p H、Mg、Ca含量呈显著正相关;酸性磷酸酶和蛋白酶酶活性与含水量、铵态氮、硝态氮、可溶性有机碳、总钾呈显著正相关,而与可溶性有机氮、p H、Mg和Ca呈显著负相关;另外,MBC和MBN与酸性磷酸酶或蛋白酶呈显著负相关,而MBC和MBN与蔗糖酶、纤维素酶或多酚氧化酶呈显著正相关;因此,松材线虫的侵染改变了松林土壤的理化性质,引起土壤微生物群落结构、生物量和土壤酶活性的变化,这些指标可用于指示和评价松材线虫侵染对土壤质量的影响。
The masson pine masson pine forest soil was used as the research object to explore the effects of different degrees of pine wood nematode infection on soil physical and chemical properties, microbial biomass and soil enzyme activities. The results showed that the total carbon, total phosphorus, total potassium, dissolved organic carbon, ammonium nitrogen, nitrate nitrogen, available phosphorus and water content showed an increasing trend with the severity of the pine nematode disease, while p H, Ca, Mg, soluble organic nitrogen, microbial biomass carbon (MBC) and microbial biomass nitrogen (MBN) decreased significantly. At the same time, the enzyme activities of sucrase, urease, cellulase and polyphenol oxidase tended to increase with the degree of infection Decreased, while the acid phosphatase and protease activity was significantly increased. The results showed that the activities of soil urease, invertase, cellulase and polyphenol oxidase and the activities of soil water, ammonium nitrogen, nitrate nitrogen, soluble organic carbon, total phosphorus, total potassium, available phosphorus and total carbon But negatively correlated with the contents of soluble organic nitrogen, p H, Mg and Ca. The activities of acid phosphatase and protease and the contents of water, ammonium nitrogen, nitrate nitrogen, soluble organic carbon and total potassium were But significantly negatively correlated with soluble organic nitrogen, p H, Mg and Ca. In addition, MBC and MBN showed a significant negative correlation with acid phosphatase or protease, whereas MBC and MBN were associated with sucrase, cellulase or more Phenoloxidase showed a significant positive correlation. Therefore, the infection of pine wood nematode changed the physical and chemical properties of pine forest soil and caused changes of soil microbial community structure, biomass and soil enzyme activity. These indicators could be used to indicate and evaluate the pine wood nematode invasion Effect of dye on soil quality.