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我们曾用多种Golgi法试染神经细胞,有时也有较好的星状胶质细胞或少突胶质细胞出现。Vogt(1981)曾指出“用较稀浓度的固定液和浸染液可获得较少的神经元”。在此启示下,我们按照Peters等(1981)报导的Golgi电镜技术中介绍的固定液,并在浸染液和硝酸银的处理方面作了些改良,从而使显示少突胶质细胞取得了较为满意的效果。现将经过改良的方法介绍如下:1.材料:小猫或幼年大鼠或小鼠的大脑皮质、脑干等,材料宜新鲜。2.灌注固定:动物用36％水合氯醛进行腹腔内注射(0.1ml/100g体重)。从左心室心尖区先灌注生理盐水约200ml,再用
We used a variety of Golgi assay of nerve cells, and sometimes better astrocytes or oligodendrocytes. Vogt (1981) pointed out that “fewer neurons can be obtained with more dilute fixative and disseminated fluids.” Inspired by this, we have made some improvements in the treatment of disseminating solution and silver nitrate according to the fixation solution described by Golgi in electron microscopy reported by Peters et al. (1981), which makes the display of oligodendrocytes more satisfactory Effect. Now improved methods are introduced as follows: 1. Materials: Kittens or young rats or mice cerebral cortex, brain stem, etc., the material should be fresh. 2. Perfusion fixation: Animals were injected ip (0.1 ml / 100 g body weight) with 36% chloral hydrate. From the left apex ventricular perfusion saline about 200ml, reuse