论文部分内容阅读
墨兰的无菌播种结果发现，在不添加细胞分裂素的培养基上，种子可以发芽，但只有原球茎和根状茎产生；不可能进一步分化成苗，只有在含有不同激素成分的MS或KnudsonC培养基上，才有可能诱导芽的分化，其中以附加6－BA0．5-1．0mg／L＋NAA0．1mg／L诱导效果最佳，在附加6－BA2．0mg／L+NAA0．4mg／L的MS培养基中，能加速芽的增殖，根状茎转入含有相同激素成分的液体增殖培养基中振荡培养，可大大提高芽的分化速率。添加0．5％活性炭对芽的分化有明显增效作用。在附加NAA0．2mg／L的MS培养基中；幼苗的生根效果最佳。
Silan’s germ-free sowing results showed that seeds could germinate on medium without cytokinin but only protocorms and rhizomes; it was impossible to further differentiate into shoots, only in MS with different hormone components or KnudsonC medium, it is possible to induce the bud differentiation with the best 6-BA0.5-1.0mg / L + NAA0.1mg / L induction effect, with additional 6-BA2.0mg / L + NAA0.4mg / L MS medium, can accelerate the proliferation of buds, rhizomes transferred to liquid growth medium containing the same hormone components in shaking culture, can greatly improve the bud differentiation rate. Addition of 0.5% activated carbon had obvious synergistic effect on bud differentiation. In addition NAA0.2mg / L of MS medium; seedling rooting best.