Expression of Mammalian P1 Protamine Gene

• 出 处: Asian Journal of Andrology
• 发表日期: 2003年01期

Objective: To study the expression of the mammalian P1 protamine gene in rat and mouse. Methods: Rat protamine (rP) cDNA was obtained by using RT-PCR technique. Mouse P1 protamie (mP1) cDNA was prepared from recombinate plasmid pUC8. Diglabelled rP cDNA and mP1 cDNA were used for Northern bolt analysis to study the expression of P1 protamine. Results: P1 protamine mRNA was detected only in rat testis, no hybridization signals were detected in rat brain and liver. In addition, the presence of P1 protamine mRNA was detected in both rat and mouse testis. In the process of sexual maturation of mouse, 7-8 d after birth, no mPl mRNA could be detected; at 24~26 d, mP1 mRNA was detectable migrating as a homogeneous band at 580 nucleotides, whereas in sexually mature animals, a heterogeneous mixture of RNAs ranging 450~580 bases in length was observed. Histological studies revealed that in the testis of 7~8-day-old mouse, spermatogenesis was developed to the spermatocyte stage, whereas round spermatids (Rs) were present in the testis of the 24~26-day-old mouse and elongating spermatids (Es) were present in the testis of sexually mature animals. Conclusion: P1 protamine gene is tissue-specifically expressed and the P1 protamine is shown to be conservative in evolution. During the process of sexual maturation, along with morphological changes, mP1 gene was transcribed in Rs and translated in Es. Objective: To study the expression of the mammalian P1 protamine gene in rat and mouse. Methods: Rat protamine (rP) cDNA was obtained by using RT-PCR technique. Mouse P1 protamie (mP1) cDNA was prepared from recombinate plasmid pUC8 . Diglabelled rP cDNA and mP1 cDNA were used for Northern bolt analysis to study the expression of P1 protamine. Results: P1 protamine mRNA was detected only in rat testis, no hybridization signals were detected in rat brain and liver. P1 protamine mRNA was detected in both rat and mouse testis. In the process of sexual maturation of mouse, 7-8 d after birth, no mP1 mRNA could be detected; at 24 ~ 26 d, mP1 mRNA was detectable migrating as a homogeneous band at 580 nucleotides, while in sexually mature animals, a heterogeneous mixture of RNAs ranging 450-580 bases in length was observed. Histological studies revealed that in the testis of 7-8-day-old mouse, spermatogenesis was developed to the spermatocyte stage, wherea s round spermatids (Rs) were present in the testis of the 24-26 day-old mouse and elongating spermatids (Es) were present in the testis of sexually mature animals. Conclusion: P1 protamine gene is tissue-specifically expressed and the P1 During the process of sexual maturation, along with morphological changes, mP1 gene was transcribed in Rs and translated in Es.