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目的检测裂解素-金属蛋白酶33基因(ADAM33)F+1位点单核甘酸多态性在支气管哮喘患者中的分布频率,并探讨其与支气管哮喘的相关性。方法采用等位基因特异性聚合酶链反应(AS-PCR)技术及DNA测序的方法,对哮喘组126例患者及对照组121例健康者进行ADAM33基因F+1位点单核苷酸多态性分析。结果 ADAM33基因F+1位点哮喘组和对照组基因型分布均符合Hardy-Weinburg定律;ADAM33基因F+1位点3种基因型(CC、CT、TT)在哮喘组分布分别为68(54.0%)、48(38.1%)、10(7.9%),在对照组分布分别为70(57.9%)、46(38.0%)、5(4.1%),无显著性差异(χ2=1.638,P>0.05)。哮喘组和对照组ADAM33基因F+1位点等位基因C和T的频率分别为0.730、0.270、0.769、0.231,两组等位基因频率比较差异无显著性(χ2=0.970,P>0.05)。结论 ADAM33基因F+1位点在支气管哮喘患者中存在CC、CT、TT多态性,ADAM33基因F+1位点基因多态性与支气管哮喘无明显相关性。
Objective To detect the frequency of F + 1 SNP polymorphism of lytic-metalloproteinase 33 gene (ADAM33) in patients with bronchial asthma and to explore its relationship with bronchial asthma. Methods Allele-specific polymerase chain reaction (AS-PCR) and DNA sequencing were used to detect the polymorphisms of F + 1 locus in ADAM33 gene in 126 patients with asthma and 121 healthy controls. Sexual analysis. Results The genotype distribution of ADAM33 gene F + 1 locus in asthma group and control group were in accordance with Hardy-Weinburg law. The distributions of CC, CT and TT in F + 1 locus of ADAM33 gene in asthma group were 68 (54.0 (Χ2 = 1.638, P> 0.05). There was no significant difference between the two groups in the control group (70%, 48%, 38% 0.05). The frequencies of allele C and T in F + 1 locus of ADAM33 gene in asthma group and control group were 0.730,0.270,0.769,0.231, respectively. There was no significant difference in allele frequencies between two groups (χ2 = 0.970, P> 0.05) . Conclusion There is polymorphism of CC, CT and TT in F + 1 locus of ADAM33 gene in patients with bronchial asthma. There is no significant correlation between polymorphism of F + 1 locus in ADAM33 gene and bronchial asthma.